Plant Physiology (Biology 327) - Dr. Stephen G. Saupe; College of St. Benedict/ St. John's University; Biology Department; Collegeville, MN 56321; (320) 363 - 2782; (320) 363 - 3202, fax; ssaupe@csbsju.edu |
Water Potential - Materials List
Plant Materials
Potatoes (or, students bring own materials)
freeze some peeled potatoes overnight
Chemicals
salt (rock or other inexpensive)
sucrose
methylene blue
Center table
Sucrose solutions (0.1 ‑ 0.8 molal; 30 mL per group plus approx. 100 mL)
0.1 molal (34.23 g added to 1000 mL water)
0.2 molal (68.46 g/1000 mL)
0.3 molal (102.70 g/1000 mL)
0.4 molal (136.94 g/1000 mL)
0.5 molal (171.17 g/1000 mL)
0.6 molal (205.40 g/1000 mL)
0.7 molal (239.64 g/1000 mL)
0.8 molal (273.84 g/1000 mL)
water
25 mL graduate cylinder to measure sucrose, place next to each container (or pipette or other)
Sucrose dyed blue with methylene blue in small bottles (each with a disposable pipette)
cork borer sharpener
balances
Freezing Point Supply Area
blender and base
clinical centrifuge
clinical centrifuge tubes & caps (1/person)
ice + bucket
rock salt
glass rod or spatula (to push potato into blender)
frozen potatoes
alcohol (denatured, 1 mL per group) � with pipette and pump
Student Tables
test tube rack (1/group)
9 tubes (microfuge or other)
spoon (1/group)
label tape
marker
weigh boat (2/group)
cork borer (1/group)
ruler
thermometer (1/group)
bamboo skewer or probe without needle to push core out of borer
kimwipes
paper towels
disposable pipettes (8/group)
Freezing point apparatus (ring stand, 600 mL beaker, low temperature thermometer in rubber stopper, 2 clamps, mini stir bar, thermometer, flat bottom tube, glass stir rod)
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Last updated:
01/07/2009 � Copyright by SG
Saupe