Plant Physiology (Biology 327) - Dr. Stephen G. Saupe; College of St. Benedict/ St. John's University; Biology Department; Collegeville, MN 56321; (320) 363 - 2782; (320) 363 - 3202, fax; ssaupe@csbsju.edu |
Determining the Frequency & Volume of Pigmented and Unpigmented Red Cabbage Protoplasts
Questions: What is the volume of a
red cabbage leaf protoplast? How much variation exists in the size of protoplasts? Are
unpigmented protoplasts the same size as pigmented ones? What percent of the red
cabbage protoplasts are pigmented?
Unpigmented? Why are some protoplasts colorless (unpigmented)?
Hypotheses: Red cabbage protoplasts will be similar in size (diameter = 80 �m; volume = 2.68 x 105 �m3) to those reported for tulip protoplasts (Wagner, 1979). The protoplasts will exhibit normal variation in size. There will be a greater number of unpigmented protoplasts since the pigment is restricted to the epidermal cells in the red cabbage leaf.
Protocol:
@ 40x 1 omu = ________ �m
@100x 1 omu = ________ �m
@400x 1 omu = ________ �m
magnification used to measure protoplasts = ______________
Data, Analysis & Conclusions:
Question: Are the pigmented and unpigmented protoplasts the same size?
Ho:
t = ________________ p = __________________Conclusion:
Table 1: Pigmented and Unpigmented Red Cabbage Protoplasts | |||||||
Field |
Total |
% Total | |||||
1 |
2 |
3 |
4 |
5 |
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Pigmented | |||||||
Unpigmented |
Table 2: Diameter and volume of pigmented red cabbage protoplasts in 0.7 M mannitol | |||||
Protoplast | Diameter (omu) | Diameter (�m) | Radius (�m) | Protoplast volume (�m3) | Protoplast volume (pl) |
1 |
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2 |
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3 |
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4 |
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5 |
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6 |
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7 |
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8 |
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9 |
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10 |
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11 |
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12 |
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13 |
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14 |
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15 |
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16 |
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17 |
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18 |
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19 |
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20 |
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21 |
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22 |
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23 |
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24 |
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25 |
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26 |
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27 |
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28 |
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29 |
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30 |
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Mean (+/- std dev) |
Table 3: Diameter and volume of unpigmented red cabbage protoplasts in 0.7 M mannitol | |||||
Protoplast | Diameter (omu) | Diameter (�m) | Radius (�m) | Protoplast volume (�m3) | Protoplast volume (pl) |
1 |
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2 |
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3 |
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4 |
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5 |
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6 |
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7 |
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8 |
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9 |
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10 |
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11 |
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12 |
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13 |
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14 |
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15 |
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16 |
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17 |
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18 |
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19 |
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20 |
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21 |
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22 |
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23 |
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24 |
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25 |
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26 |
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27 |
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28 |
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29 |
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30 |
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Mean (+/- std dev) |
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Last updated:
01/07/2009 � Copyright by SG
Saupe