Concepts of Biology (BIOL115) - Dr. S.G. Saupe (ssaupe@csbsju.edu); Biology Department, College of St. Benedict/St. John's University, Collegeville, MN 56321 |
Energy - Releasing Pathways
I. Biological Energetics: A Brief Primer
What is energy? A simple definition - energy is the ability
to do work (which is the same as moving matter). Organisms need to do a lot of
work (i.e., metabolism, membrane transport, movement). Measured in
units of kJ (kilojoule) or kcal (kilocalorie - this is the traditional
unit and less preferred than kJ since it is a unit of heat. It is used
since all work can essentially be converted to heat)
II. Reducing Potential - ability of a substance to participate in a redox reaction. Living organisms must carry out many redox reactions.
→ NADH + H+NAD+ + 2H+ + 2e-
III. Chemical Potential - energy available from bond cleavage.
The primary source is ATP (adenosine triphosphate), which is
the energy currency of life. If a cell or organism wants to get some
"work" done, it "pays" for the work with ATP. It is
estimated that we use and cycle approximately a body weight worth of ATP
everyday.
IV. Equation Review.
Given that background, let�s review the equations for
photosynthesis and respiration that you�ve seen many times:
photosynthesis: | CO2 + H2O + light energy → (CH20)n + O2 |
respiration: | (CH2O)n + O2 → CO2 + H2O + chemical energy |
Now, let's look at some exciting details!
- Anabolism is analogous to pushing the rock uphill, catabolism is analogous to the rock rolling downhill;
- Photosynthesis (an anabolic process) is analogous to pushing the rock uphill, respiration (a catabolic process) is analogous to the rock rolling downhill;
- The energy required to push the rock (glucose) uphill comes from light (radiant energy);
- The release of energy from glucose rolling downhill is coupled to ATP production (ca. 40% of the energy is trapped in ATP but more than half of the energy is lost as heat).
VI. Rolling metabolic rocks downhill - A look at Glucose Catabolism
VII. Glycolysis - the first steps of glucose catabolism.
VIII. Fermentation - stepping in an anaerobic environment
IX. Aerobic or Cellular Respiration - stepping in air.
- It is named in honor of Sir Hans Krebs, who owned a bicycle shop in Kent, England. Just kidding - he was a British biochemist who worked out the details of many of the reactions. It is also called "Citric Acid Cycle" because citric acid is one of the important intermediate molecules or called the "Tricarboxylic Acid Cycle" (TCA) because citric acid has three carboxyl groups. Take your pick of names.
- Occurs in the matrix of the mitochondrion (thus the reactants are water soluble)
- There are several different reactions in the Citric Acid cycle, each catalyzed by a different enzyme.
- There is one substrate level phosphorylation reaction that produces ATP. [Actually, the first product of the cycle is GTP (which is a nucleotide phosphate carrier like ATP). The GTP then donates a phosphate to ADP to make ATP.]
- Carbon dioxide is released during two reactions (alpha-ketoglutarate dehydrogenase and isocitrate dehydrogenase) (or three total reactions if you include the reaction during which pyruvate was shuttled into the mitochondrion). Thus, during the Citric Acid cycle, the breakdown of glucose into carbon dioxide is completed.
- There are four redox reactions, three of which yield reduced NADH and one FADH2. Thus, the oxidation of glucose is completed in the Kreb's cycle. If you count the redox reaction that occurred when shuttling pyruvate into the mitochondrion there is a total of 5 redox reactions in the mitochondrion.
X. Regenerating Coenzymes in aerobic conditions
Recall that one problem of glycolysis was
regenerating oxidized coenzymes. Under anaerobic conditions, this problem was
solved by a variety of fermentation reactions or anaerobic respiration. Well,
the solution is much more elegant in cells in an aerobic environment. Not only
are oxidized coenzymes recovered, but the process is coupled to the production
of ATP. Thus, the evolution of an oxygenated atmosphere allowed cells the added
bonus of producing additional cellular energy while regenerating oxidized
coenzymes. The electron transport chain (ETC) provided the means for this
process.
XI. Mitochondrial Electron Transport Chain
� III � IV
- There are four major groups, called complexes, of electron carriers in the membrane. This is the reason why the text shows several blobs in the membrane. Each complex has a unique set of carriers. In addition, there are molecules that shuttle electrons from one complex to another.
- 2. Among the carriers in the complexes (don't memorize these): Complex I - flavoproteins (FMN) and Fe-S proteins; Complex II - more flavoproteins, Fe-S proteins; Complex III - an assortment of proteins including cytochromes (c1 and b); Complex IV - cytochrome a,a3.
- The sequence of electron flow occurs from complex I to complex IV as depicted: I
→ Q → III → cyt c → IVI
NADH → I → Q → III → cyt c → IV
NADH → I → Q → III → cyt c → O2
FADH2/NADHex → II → Q → III → cyt c → IV → O2
D. The passage of electrons along the ETC is associated with the production of ATP.
XII. Chemiosmotic Production of ATP
XIII. Function of Aerobic Respiration/Glucose Catabolism
XIV. Regulation
Many of the enzymes of glycolysis and respiration are
allosteric enzymes. This allows for tight control of metabolism.
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